Journal: Microbiology and Immunology

Article Title: Application of an enzyme‐labeled antigen method for visualizing plasma cells producing antibodies against Strep A, a carbohydrate antigen of Streptococcus pyogenes , in recurrent tonsillitis

doi: 10.1111/1348-0421.12213

Figure Lengend Snippet: Summary of clinical data and analyzed results in 12 cases of recurrent tonsillitis

Article Snippet: Three rats were immunized with boiled S. pyogenes (strain: J17A4, serotype: M6, inoculum size: 4 × 10 6 CFU per rat, supplied by Eiken Chemical, Nogi, Tochigi, Japan) emulsified with Freund's incomplete adjuvant (Difco Laboratories; Detroit, MI, USA).

Techniques:

Journal: Microbiology and Immunology

Article Title: Application of an enzyme‐labeled antigen method for visualizing plasma cells producing antibodies against Strep A, a carbohydrate antigen of Streptococcus pyogenes , in recurrent tonsillitis

doi: 10.1111/1348-0421.12213

Figure Lengend Snippet: Number and percentage of plasma cells with anti‐Strep A reactivity in the axillary and popliteal lymph nodes of S. pyogenes ‐immunized and non‐immunized rats

Article Snippet: Three rats were immunized with boiled S. pyogenes (strain: J17A4, serotype: M6, inoculum size: 4 × 10 6 CFU per rat, supplied by Eiken Chemical, Nogi, Tochigi, Japan) emulsified with Freund's incomplete adjuvant (Difco Laboratories; Detroit, MI, USA).

Techniques: Clinical Proteomics

Journal: Infectious Disease Clinics of North America

Article Title: USE OF THE CLINICAL MICROBIOLOGY LABORATORY FOR THE DIAGNOSIS AND MANAGEMENT OF INFECTIOUS DISEASES RELATED TO THE ORAL CAVITY

doi: 10.1016/S0891-5520(05)70108-2

Figure Lengend Snippet: LABORATORY DETECTION OF ORAL MICROBIAL PATHOGENS

Article Snippet: In a national survey, the College of American Pathologists (CAP) sent 2692 laboratories a swab seeded with S. pyogenes to perform a rapid detection test with the kit used at their institution.

Techniques: Infection, Virus, Diagnostic Assay

Journal: Nature Communications

Article Title: Glycosylation of serine/threonine-rich intrinsically disordered regions of membrane-associated proteins in streptococci

doi: 10.1038/s41467-025-58692-8

Figure Lengend Snippet: a – c Representative TLC analysis of [ 3 H]Glc-lipids extracted from in vitro incubations of UDP-[ 3 H]Glc with the membrane fractions isolated from S pyogenes WT ( a ), Δ gtrB ( b ), and Δ gtrB :p gtrB ( c ). d Coomassie-stained gel of S. pyogenes membrane proteins purified by conA affinity chromatography. Proteins in excised bands were identified by LC-MS/MS analysis as described in Methods. The major identified proteins are indicated. e , f Immunoblot analysis of PrsA1 ( e ) and PknB ( f ) in S. pyogenes WT, Δ gtrB , Δ gtrB :p gtrB , and Δ gtrB :p sccN using specific antibodies. Anti-PrsA1 antibodies also recognize PrsA2. Proteins were separated on a 4-12% SurePAGE™– Bis-Tris gel (Genscript) in MES buffer in d – f . The experiments were performed independently three times in a , b , c , e , and f , and two times in d , yielding the same results. A representative image from one experiment is shown. g Topology of extracytoplasmic domains of S. pyogenes conA-bound proteins. * The cytoplasmic domain of PknB is omitted for clarity. Just one monomer of the dimer for PrsA1 and PknB is shown for clarity. PrsA1 is depicted as a diacylated lipoprotein because lipoproteins in streptococci are diacylated as N-acyl-glyceryl-cysteine , . Source data for a – f are provided as a Source Data file. Schematic in g was created in BioRender .

Article Snippet: Rabbit polyclonal antibodies against the S. pyogenes PrsA1 (1:1000) and PrsA2 (1:1000) homologs were provided by Dr. Yung-Chi Chang .

Techniques: In Vitro, Membrane, Isolation, Staining, Purification, Affinity Chromatography, Liquid Chromatography with Mass Spectroscopy, Western Blot